CELLINK Lumen X

The launch of the Lumen X was the culmination of stereolithography and 3D bioprinting expertise, developed and commercialized.

The Lumen X has been established as a complementary product to the Holograph X and BIO X 3D bioprinters also as all bio-inks within CELLINK’s portfolio for 3D Printing services. It’s said to leverage over a million points of light to bio-print microscopic features right down to 200 microns.

This platform also photographically cures entire layers directly, crosslinking structures fifty times faster than alternative bioprinting methods for online 3D Printing services.

The Lumen X is furthermore capable of printing living cells within a BIO X-fabricated structure which accelerates organ-on-a-chip research which is a very wonderful quality of a printer.

This bio 3D printer is often used for applications in microfluidics, macroporous structure, cell-laden hydrogels, and more.

Lumen X uses SLA technology together with patent-pending bioinks to permit scientists new tools for detailed research in futuristic tissue engineering with the help of 3D Printing services. 

Remarkable for the bioprinting industry, the Lumen X is being marketed as entry-level hardware, meant to enrich the recently released Holograph X, which may print features as complex as 10 microns for complex, advanced needs within the laboratory. 

The BIO X is another complementary hardware product, amid the expansive CELLINK bio-ink portfolio for 3D Printing services in India.

The Lumen X leverages a biocompatible blue light-based curing system and was designed to bioprint vasculature. Ti enables high resolution, high throughput, and hi-fi prints, enhancing applications in microfluidics, cell-laden hydrogels, macroporous structures and more.

 Lumen X also can represent a big advantage in achieving complex branching and tapering of vessels, constructing microscopic features with speed, fidelity, and precision.

Working with CELLINK on technology and market reach means the Lumen X is going to be ready to combine with CELLINK’s BIO X to multiply the advantages of every system’s state-of-the-art capabilities. 

Researchers are going to be ready to use Lumen X to print living cells within a BIO X-fabricated structure, thus strengthening their add applications like organ-on-a-chip and multilateral research with 3D Printing online.

The Printing Principle

The Lumen X builds solid hydrogels by beginning with a droplet of light sensitive, liquid PhotoInk™ in a vat. An industrial blue-light projector exposes a series of images onto the vat, like a slideshow. The areas of the droplet that are exposed will crosslink and solidify into a single layer. The build platform moves up to allow each layer to stack and build the part. 

Design and Specification

• Projection stereolithography: Over 1 million simultaneous points of light.
• Projected image: 1280 x 800 pixels.
• Pixel resolution (XY): 50 μm.
• Z-precision (motor-driven): 5 μm.
• Max build volume of the printer: 65 x 35 x 50 mm.

Projected light

• Biocompatible wavelength: 405 nm.
• Intensity Range: 10 – 30 mW/cm².
• Distortion: but one per cent.

Additional features

• Disposable, quick-change sterile vats.
• Heated platform: up to 37° C.
• Technology and hardware
• Software
• Seamless, intuitive workflow.
• Capacitive touch interface
• Compatible file type: STL (stereolithography) file.
• Electrical
• Power input: 100 – 265 VAC, 50 – 60 Hz, 100 W.
• Size and weight
• Dimensions: 24 x 43 x 41 cm (9.5 x 17 x 16.5 in).
• Weight: 9 kilograms (20 pounds).

Features

SPEED, FIDELITY, AND PRECISION

Lumen X gives you a strong advantage in achieving complex branching and tapering of vessels as it is designed to bioprint vasculature with biocompatible blue light.

Leverages over 1 million points of light to bioprint microscopic features right down to 200 microns.

Photographically cures entire layers directly to crosslink structures fifty times faster than other printing methods.

BIO X will be used in the due course to print living cells within a Lumen X-fabricated structure and strengthen applications like organ-on-a-chip and multi-material research.

Print Quality

Stereolithography (SLA) processes, light-based bioprinting produces constructs by initiating chemical reactions that solidify are adapted

The 2 sorts of light-based technologies, digital light processing (DLP) and holographic, are much faster because they cure whole layers and even blocks of bioinks simultaneously. 

Because of small points of light within the millions, light-based bioprinters also are ready to recreate more intricate details at much higher resolutions. 

Software

The software used by Lumen X is LightField.

LightField is a superb solution for multi-user facilities also as single-user labs. The platform places each user’s hardware and software configurations and constitutes its own features, accordingly, displaying all relevant tools via an intuitive graphical interface. 

The IntelliCal option provides up to 10x greater accuracy across the whole focal plane than competing routines. A replacement USB-powered Hg/Ne/Ar emission line source, available separately, works seamlessly with Princeton Instruments’ hardware and software to ensure the very best possible calibration accuracy.

LightField is right for applications like optical imaging, Raman spectroscopy, fluorescence spectroscopy, laser-induced breakdown spectroscopy, photoluminescence spectroscopy, surface and materials analysis, combustion studies, and astronomy. 

No matter the application, LightField offers truly remarkable regions of interest (ROI) capabilities for 3D Printing services in India. 

The LightField software platform is fully compatible with 64-bit Microsoft® Windows® 7 and Windows Vista® operating systems. A 64-bit, event-driven API is provided for programmers.

Highlights

• Biocompatibility

Lumen X PhotoInks are biocompatible, so cells can be used with printed constructs. When using GelMA, cells can even be mixed into the PhotoInk. The built-in heater keeps GelMA liquefied and improves cell viability within the PhotoInk.

• Flexibility

Combining the Lumen X with CELLINK’s BIO X™ lets users of 3D Printing companies multiply the benefits of each system’s state-of-the-art capabilities. For example, the BIO X can print living cells within a structure fabricated on the Lumen X to strengthen work in applications like multi material research with 3D Printing online.

• Isotropy

Compared to extruded scaffolds, Lumen X scaffolds are significantly more isotropic, allowing users to control mechanical properties with geometry in all dimensions. Users can also build unique structures, such as Schwarz lattices or auxetic structures at a good 3D Printing quote.

• Resolution

DLP technology coupled with Volumetric PhotoInks allow the Lumen X to build watertight channels in hydrogels with greater consistency and precision than extrusion. This allows the Lumen X to create microfluidic lab-on-a-chip devices, strengthening work in: 

• Disease pathology 
• Drug screening
• Tissue engineering
• And more!

Open Materials Platform

The Lumen X allows users of 3D Printing Bangalore to develop and use their own materials without workarounds or extra fees, opening the door for the development of materials with unique properties or for novel tissue engineering applications.

• Speed

By curing entire layers simultaneously, the Lumen X can build structures 10 mm tall in GelMA in 30 minutes (up to 50 times faster than other printing methods). This translates to better cell viability and higher throughput when model building.

• Clarity

The photo-absorbing dye that gives the PhotoInks their color will wash out of printed structures in a matter of hours, allowing structures to be imaged via bright-field or fluorescence imaging techniques.

• Intricacy

Gentle separation forces and material strength allow the Lumen X to build intricate scaffold geometries that include porosity and vasculature in X, Y and Z dimensions. Scaffolds can now have geometry that is far more biomimetic when engineering tissue such as trabecular bone. 

3D Bioprinting

Unlike a traditional 2D printer, which prints on a flat surface, 3D printers add another dimension – depth. Known as additive manufacturing, 3D printing in Bangalore distributes different materials up and down, left and right, back and forward, to print an item later by layer.

Bioprinting refers to 3D printers which deposit layers of biomaterial to build complex bodily structures like skin, bones and even corneas. The requisite cells are taken from a patient – or, if this isn’t possible, adult stem cells can be used – and cultivated into a bioink to ‘print’ an organic object. These are typically held together through some sort of dissolvable gel or collagen scaffold which can support the cells and mold them into the correct shape.

Printing body parts may well be the next step in organ transplantation – harvesting stem cells from a transplant recipient and printing them into a replacement organ could help bypass complications associated with organ transplant such as long waits for a suitable donor or immune rejection of the new organ.

Here is a look at some of the biggest breakthroughs in 3D bioprinting, and the various techniques used to create different body parts with 3D Printing services in Chennai.

• Bones: a regenerative structure for long-term healing

A team from Swansea University in the UK has developed a bioprinting process which can create an artificial bone matrix, using durable, regenerative biomaterial.

Currently, extremely complex bone fractures are treated through a surgical procedure called bone grafting, which replaces missing or damaged bones with synthetic, cement-based materials. However, this technique of 3D Printing in Chennai comes with its limitations, as these structures can often have inappropriate mechanical integrity and don’t allow the formation of new bone tissues.

The bioprinted bones can be printed in the exact structure needed with a durable and regenerative biomaterial. This material is made from gelatin, agarose, collagen alginate, calcium phosphate and polycaprolactone.

The bioprinted bone material is capable of fusing with a patient’s natural bones over time, eventually being replaced by them.

• Corneas: a boost for biocompatibility

3D printed artificial corneas have been developed by a group of researchers in South Korea. The prototype corneas have been printed from biocompatible decellularized corneal stroma and stem cells.

The team behind the corneas hopes to see its invention replace the use of donor and synthetic corneas in surgery for cataracts and other sight complications.

Artificial corneas currently on the market are made up of recombinant collagen or chemical substances like synthetic polymer, which means they can often resist full incorporation into the eye or are not transparent after transplant.

The 3D printed corneas are constructed to mimic the lattice pattern of collagen fibrils within natural corneas by using the shear stress generated by the frictional force of the 3D printing Chennai. Regulating the shear stress allowed the researchers to control the pattern in which the fibrils were printed, meaning they could make sure these artificial corneas adequately reflected the structure of the native human cornea.

• Cartilage: revolutionizing joint care

Researchers at an Australian bio fabrication centre called BioFAB3D have built a handheld cartilage printing device called the Bio Pen with the help of 3D Printing services Mumbai.

The Bio Pen is filled with stem cells derived from a patient’s fat, which can create and surgically implant custom scaffolds of living material into failing joints. Much like 3D printed bones, the cartilage undergoes a process of growth and development within the body. So far, it’s only been tested on sheep, but its developers of 3D Printing Mumbai hope that in future the Bio Pen can help to accelerate the regeneration of functional cartilage in human patients.

In practice, a surgeon will be able to use affordable 3D Printing services of the handheld stylus to place the bioink into a damaged joint layer by layer. The ink layers, which are composed of stem cells and specially selected growth factors within a biopolymer matrix, form a structure which a patient’s reproducing cells will rapidly strengthen for lasting cartilage repair.

• Hearts: beating the functionality challenge

A group of scientists at the American Friends of Tel Aviv University have 3D printed a fully vascularized heart using fat tissue cells from a donor with the help of 3D Printing service in India.

The fat cells were partially cultured and reprogrammed into heart cells. The entire heart structure is present with all cells, blood vessels, ventricles, and chambers. The structure was based on medical images of the donor patient’s own heart.

The technology of online 3D Printing India is still in its early stages – the heart the researchers have printed is the size of a rabbit’s heart and is unable to pump any blood. The researchers of 3D Printing companies hope to test these printed hearts in animals once they have worked on rescaling the organs and getting them to beat.

• Skin: a great leap in grafting for burn patients

Wake Forest School of Medicine has designed a printer that can print skin cells directly on to a burn wound with 3D Printing online.

The traditional treatment for severe burns is skin grafting, where healthy skin is harvested from an unburned part of a patient’s body. This in itself can be traumatic to heal from, and in some cases, there isn’t enough healthy skin left on the body to use.

In this technique to 3D Print online, a patch of skin only 10% of the size of the burn can be used to grow enough cells for 3D printing Bangalore. A scanner is used to determine the size and depth of the wound, and the printer then takes this information and prints hypodermic, dermic, and epidermal skin cells at the corresponding depths to cover the wound.

As trials around this technology move forward, the research team is hoping to see if stem cells from amniotic fluid and placentas are as effective as patient skin in healing these wounds.

Conclusion

The bio printers have a lot of potential in them in the medical and biological field. The 3D printers are already being used for printing medical equipment while on the other side Bio- Printers will help in cumulative production of living cells and tissues.  

This will change the way people see the world. Now living cells can be “printed” using light and printing methods. This is one of the greatest achievements of mankind.